Iraqi Journal of Embryos and Infertility Researches

The Effect of BMI on Serum and Follicular Fluid Level of Kisspeptin in Infertile Women

Ghada Alanssari — 2020-08-08

Kisspepetin is considered as a key regulator of reproduction, through its direct role in controlling the hypothalamic pituitary gonadal axis necessary for controlling the ovulatory process and indirectly by impacting glucose heamostasis and metabolism. To study the correlation between body mass index and level of Kisspeptin in serum and follicular fluid. This study is a cross sectional study done on (80) infertile Iraqi patients aged (18-40 years) scheduled for fresh intracytoplasmic sperm injection cycle. This study is conducted in the High Institute for Infertility Diagnosis and Assisted Reproductive Technologies of Al-Naharin University, from September 2020 to May 2021. For each participant, the GnRH antagonist protocol was used to hyperstimulate the ovaries, and samples from serum and follicular fluid were obtained at day of oocytes retrieval for measuring Kisspeptin level using enzyme linked immunosorbent assay (ELIZA) technique. Serum and follicular fluid Kisspeptin were insignificantly increasing with the increase in the BMI (p value =0.406) (p value = 0.352) respectively. Serum and follicular kisspetin were positively and significantly correlated (r = 0.536, p value= 0.000). Serum Kisspeptin and follicular Kisspeptin level showed no significant difference among different BMI categories in infertile females undergoing fresh ICSI cycle. Serum Kisspeptin was significantly correlated with follicular fluid Kisspeptin.

DFI and Chromatin Maturity Status Could be Considered as Independent Parameters for Male Fertility Assessment

Huda Abdalrazaq — 2020-08-08

Human sperm chromatin and integrity of DNA have multiple endogenous factors, such as sperm compression and nucleoproteins, with various mechanisms all through their sperm production and transport. The integrity of the paternal genetic material in both regular and assisted pregnancy is essential for initiating and maintaining a viable pregnancy. This study included semen samples from 100 men taken from Al Nahrain University Medical Clinic and Babylon Teaching Hospital for Women and Children. The first sperm test was based on the World Health Organization (1999), followed by a DNA integrity test that uses a DNA fragmentation test (DFI), and the third intensification test Chromatin by aniline blue dye. It was correlated negatively with the sperm count in combination with DFI and chromatin sperm with semen characteristics (r = - 0.223, p = 0.026). As well as DFI, sperm chromatin was being clinically significant associated with the rate of round cells (r = 0.39, < 0.001). DFI was positive and significantly associated with sperm chromatin in all infertile men (r = 0.958; p<0.001). DNA isolation in all enrolled men has been adversely linked with sperm/ejaculation, has been associated with the round cell count positive way and is not understanding and resolving with other parameters. The width of abstinence and beneficial cell count in all register men were strongly linked with Sperm chromatin. There was a direct relationship between the fragmentation of DNA and chromatin. This could imply that one exam can be used instead of the other.

Effects of Activin A Hormone on Fertilization Capacity of PCOS Women Undergoing ICSI Cycle in Sample of Iraqi Women

Ghofran Khalial — 2020-08-08

Activin is a member of the transforming growth factor-beta superfamily and is a polypeptide growth factor. Activin regulates follicle growth, gonadotropin responsiveness, steroidogenesis, oocyte maturation, ovulation, and corpus luteum function in the ovary via a local autocrine/paracrine effect. Activin measurement A level in serum and follicular fluid during the preovulatry stage in PCOS women undergoing an IVF/ICSI cycle to predict fertilization capacity earlier Fifty infertile couples, 25 with PCOS and 25 without PCOS, who attended Al Nahrain University in Baghdad, Iraq's High Institute of Infertility Diagnosis and Assisted Reproductive Technologies. The females that participated were between the ages of 18 and 40. On the day of oocyte retrieval, the level of Activin A in serum and follicular fluid was measured. Activin A levels in PCOS women's serum (activin A=1274 77.9) and follicular fluid (activin A =1038 50.3) compared to non-PCOS women's serum (activin A=453 25.74) and follicular fluid (Activin A=450 23.73). The study shows no correlation between activin A hormone with oocytes characteristics and fertilization rate in PCOS women with a significant increase of activin A hormone in the serum and FF of PCOS women compared to non PCOS women. Activin A level can predict a diagnosis of PCOS in addition to positive predictive pregnancy but not indicator of fertilization capacity in PCOS women.

Intra cytoplasmic sperm injection (ICSI) outcome comparison among normal, poor and hyper responder females following controlled ovarian stimulation using GnRH antagonist protocol.

Muhjah Falah — 2020-08-08

The aim of any given ICSI cycle is to achieve normal response and avoid poor
or hyper response. A part from ovarian hyper stimulation syndrome (OHSS),
pregnancy rate in hyper responder females usually low which could be related
to the adverse effect of hyper estrogenic state on oocytes, embryos quality and
uterine endometrium. To evaluate the effect of hyper response on ICSI
outcome represented by oocytes, embryos quality, fertilization and pregnancy
rates. Study showed that total number of retrieved oocytes, mature, immature
oocytes, total embryos, good quality embryos and blastocyst count were
significantly higher in hyper followed by normal and poor responders
respectively. Fertilization rate was significantly higher in both hyper and
normal responders than poor ones, while pregnancy rate was significantly
higher in hyper responders 47.6% followed by normal 40.7% and then poor
ones 16.7%. Conclusions: Irrespective of abnormal hormonal milieu,
excessive ovarian response and higher number of produced oocytes improve
ICSI outcome in hyper responder females by increasing the chance of getting
higher number of embryos, good quality embryos and even blastocyst
numbers which in turn increase the chance of pregnancy

Correlation between post-tamoxifen hormonal changes and seminal fluid analysis parameters improvement

Ali Abdulkhaleq — 2020-08-08

Oligoasthenoteratozoospermia is a prevalent cause of male infertility.
(iOAT). It is generally acknowledged that, due to the fact that
spermatogenesis is a sex-hormone-dependent process, specific endocrine
treatments might enhance or recover male fertility. The typical negative
feedback of sex steroids is disrupted by anti-estrogen medication, leading to
a rise in endogenous gonadotropin-releasing hormone secretion (FSH and
LH) straight from the pituitary. The stimulation of Leydig cells in the testes
by FSH and LH is thought to promote local testosterone synthesis, which in
turn may improve spermatogenesis. The study sample consisted of 42 iOAT
patients. Baseline seminal fluid analysis (SFA) and hormonal assessment
(FSH, LH, prolactin, and testosterone) were taken. Each patient was given
medical treatment with tamoxifen (20 mg/day) for three months. SFA, FSH,
and testosterone were taken after three months. There was a significant
increase in FSH, testosterone, sperm concentration, and progressive motility
after treatment (p value <0.001), furthermore, there was no significant
correlation between hormonal changes and changes in SFA study markers
(p value > 0,05). Improvements in hormone levels following tamoxifen
treatment for patients with idiopathic oligoasthenoteratospermia do not
correlate with alterations in SFA markers.