Iraqi Journal of Embryos and Infertility Researches <div class="CjVfdc">The Iraqi Journal of Embryos and Infertility Researches (IJEIR) is the first Iraqi journal in the field of infertility, clinical and applied embryology and Assisted Reproductive Technologies (ART's). It is the official journal of the institute of embryos researches and infertility treatment (the former name of the Institute), the High Institute of Infertility Diagnosis and Assisted Reproductive Technologies (current name) in Al Nahrain University. The first issue was released in 2011 after including in the Global Index for journals (ISSN: 2218 -0265). It has also been documented in the Iraqi National Library with an archive deposit number:1426-2010. The journal publishes articles and researches in the field of fertility, reproduction, special embryology and reproductive genetics fields. It is a peer reviewed journal, which specializes in providing the possibility for researchers to publish research, clinical and academic articles specialized in reproduction and infertility to humans, and animals. The journal covers a large area of scientific topics: Special Science of reproduction including physiology, pathology, urinary, science of cells, tissues cellular genetics and others. The journal publishes scientific articles in accordance with international standards and based on the evaluator scientific specialists inside and outside Iraq. It is issued twice a year to stay with a high and influential scientific level so topped the journal within prime location between scientific journals published by Al Nahrain University establishment. The journal is indexed in the Crossref and Iraqi Academic Scientific Journals. In addition to Publons website.</div> The High Institute for Infertility Diagnosis and Assisted Reproductive Technologies en-US Iraqi Journal of Embryos and Infertility Researches 2218-0265 <div class="CjVfdc">All articles are licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.</div> <div id="h.3b688g5i3sox" class="GV3q8e aP9Z7e"> </div> <div class="CjVfdc"> <div class="PPhIP rviiZ"> <div class="U26fgb mUbCce fKz7Od LRAOtb Znu9nd M9Bg4d" role="presentation" aria-describedby="h.3b688g5i3sox" aria-label="Copy heading link" aria-disabled="false" data-tooltip="Copy heading link" aria-hidden="true" data-tooltip-position="top" data-tooltip-vertical-offset="12" data-tooltip-horizontal-offset="0"> <div class="VTBa7b MbhUzd"> </div> </div> </div> <span class=" OGiC0d aw5Odc"><a class="XqQF9c" href=";sa=D&amp;sntz=1&amp;usg=AFQjCNHR4439nM4_Ar7-II5_R_iZxNxJdg" target="_blank" rel="noopener"><strong></strong></a></span></div> The Effect of BMI on Serum and Follicular Fluid Level of Kisspeptin in Infertile Women <p>Kisspepetin is considered as a key regulator of reproduction, through its direct role in controlling the hypothalamic pituitary gonadal axis necessary for controlling the ovulatory process and indirectly by impacting glucose heamostasis and metabolism. To study the correlation between body mass index and level of Kisspeptin in serum and follicular fluid. This study is a cross sectional study done on (80) infertile Iraqi patients aged (18-40 years) scheduled for fresh intracytoplasmic sperm injection cycle. This study is conducted in the High Institute for Infertility Diagnosis and Assisted Reproductive Technologies of Al-Naharin University, from September 2020 to May 2021. For each participant, the GnRH antagonist protocol was used to hyperstimulate the ovaries, and samples from serum and follicular fluid were obtained at day of oocytes retrieval for measuring Kisspeptin level using enzyme linked immunosorbent assay (ELIZA) technique. Serum and follicular fluid Kisspeptin were insignificantly increasing with the increase in the BMI (p value =0.406) (p value = 0.352) respectively. Serum and follicular kisspetin were positively and significantly correlated (r = 0.536, p value= 0.000). Serum Kisspeptin and follicular Kisspeptin level showed no significant difference among different BMI categories in infertile females undergoing fresh ICSI cycle. Serum Kisspeptin was significantly correlated with follicular fluid Kisspeptin.</p> Ghada Alanssari Mufeda Jwad Amal Mohammed Copyright (c) 2020 Ghada Alanssari, Mufeda Ali Jwad, Amal Abdulwahid Mohammed 2020-08-08 2020-08-08 10 2 75 90 10.28969/IJEIR.v10.i2.r5.20 DFI and Chromatin Maturity Status Could be Considered as Independent Parameters for Male Fertility Assessment <p>Human sperm chromatin and integrity of DNA have multiple endogenous factors, such as sperm compression and nucleoproteins, with various mechanisms all through their sperm production and transport. The integrity of the paternal genetic material in both regular and assisted pregnancy is essential for initiating and maintaining a viable pregnancy. This study included semen samples from 100 men taken from Al Nahrain University Medical Clinic and Babylon Teaching Hospital for Women and Children. The first sperm test was based on the World Health Organization (1999), followed by a DNA integrity test that uses a DNA fragmentation test (DFI), and the third intensification test Chromatin by aniline blue dye. It was correlated negatively with the sperm count in combination with DFI and chromatin sperm with semen characteristics (r = - 0.223, p = 0.026). As well as DFI, sperm chromatin was being clinically significant associated with the rate of round cells (r = 0.39, &lt; 0.001). DFI was positive and significantly associated with sperm chromatin in all infertile men (r = 0.958; p&lt;0.001). DNA isolation in all enrolled men has been adversely linked with sperm/ejaculation, has been associated with the round cell count positive way and is not understanding and resolving with other parameters. The width of abstinence and beneficial cell count in all register men were strongly linked with Sperm chromatin. There was a direct relationship between the fragmentation of DNA and chromatin. This could imply that one exam can be used instead of the other.</p> Huda Abdalrazaq Ali Rahim Laith Abd AL- Hussein Copyright (c) 2020 Huda Abdalrazaq, Ali Ibrahim Rahim, Laith Amer Abd AL- Hussein 2020-08-08 2020-08-08 10 2 107 115 10.28969/IJEIR.v10.i2.r7.20 Effects of Activin A Hormone on Fertilization Capacity of PCOS Women Undergoing ICSI Cycle in Sample of Iraqi Women <p>Activin is a member of the transforming growth factor-beta superfamily and is a polypeptide growth factor. Activin regulates follicle growth, gonadotropin responsiveness, steroidogenesis, oocyte maturation, ovulation, and corpus luteum function in the ovary via a local autocrine/paracrine effect. Activin measurement A level in serum and follicular fluid during the preovulatry stage in PCOS women undergoing an IVF/ICSI cycle to predict fertilization capacity earlier Fifty infertile couples, 25 with PCOS and 25 without PCOS, who attended Al Nahrain University in Baghdad, Iraq's High Institute of Infertility Diagnosis and Assisted Reproductive Technologies. The females that participated were between the ages of 18 and 40. On the day of oocyte retrieval, the level of Activin A in serum and follicular fluid was measured. Activin A levels in PCOS women's serum (activin A=1274 77.9) and follicular fluid (activin A =1038 50.3) compared to non-PCOS women's serum (activin A=453 25.74) and follicular fluid (Activin A=450 23.73).&nbsp; The study shows no correlation between activin A hormone with oocytes characteristics and fertilization rate in PCOS women with a significant increase of activin A hormone in the serum and FF of PCOS women compared to non PCOS women. Activin A level can predict a diagnosis of PCOS in addition to positive predictive pregnancy but not indicator of fertilization capacity in PCOS women.</p> Ghofran Khalial Wasan Abdulhameed Saad Al-Dujaily Copyright (c) 2020 Ghofran Khalial, Wasan Adnan Abdulhameed, Saad S. Al-Dujaily 2020-08-08 2020-08-08 10 2 14 24 10.28969/IJEIR.v10.i2.r2.20 Intra cytoplasmic sperm injection (ICSI) outcome comparison among normal, poor and hyper responder females following controlled ovarian stimulation using GnRH antagonist protocol. <p>The aim of any given ICSI cycle is to achieve normal response and avoid poor <br />or hyper response. A part from ovarian hyper stimulation syndrome (OHSS), <br />pregnancy rate in hyper responder females usually low which could be related <br />to the adverse effect of hyper estrogenic state on oocytes, embryos quality and <br />uterine endometrium. To evaluate the effect of hyper response on ICSI <br />outcome represented by oocytes, embryos quality, fertilization and pregnancy <br />rates. Study showed that total number of retrieved oocytes, mature, immature <br />oocytes, total embryos, good quality embryos and blastocyst count were <br />significantly higher in hyper followed by normal and poor responders <br />respectively. Fertilization rate was significantly higher in both hyper and <br />normal responders than poor ones, while pregnancy rate was significantly <br />higher in hyper responders 47.6% followed by normal 40.7% and then poor <br />ones 16.7%. Conclusions: Irrespective of abnormal hormonal milieu, <br />excessive ovarian response and higher number of produced oocytes improve <br />ICSI outcome in hyper responder females by increasing the chance of getting <br />higher number of embryos, good quality embryos and even blastocyst <br />numbers which in turn increase the chance of pregnancy</p> Muhjah Falah Wasan A. Abdulhameed Copyright (c) 2020 Muhjah Falah, Wasan A. Abdulhameed 2020-08-08 2020-08-08 10 2 51 74 10.28969/IJEIR.v10.i2.r4.20 Correlation between post-tamoxifen hormonal changes and seminal fluid analysis parameters improvement <p>Oligoasthenoteratozoospermia is a prevalent cause of male infertility. <br />(iOAT). It is generally acknowledged that, due to the fact that <br />spermatogenesis is a sex-hormone-dependent process, specific endocrine <br />treatments might enhance or recover male fertility. The typical negative <br />feedback of sex steroids is disrupted by anti-estrogen medication, leading to <br />a rise in endogenous gonadotropin-releasing hormone secretion (FSH and <br />LH) straight from the pituitary. The stimulation of Leydig cells in the testes <br />by FSH and LH is thought to promote local testosterone synthesis, which in <br />turn may improve spermatogenesis. The study sample consisted of 42 iOAT <br />patients. Baseline seminal fluid analysis (SFA) and hormonal assessment <br />(FSH, LH, prolactin, and testosterone) were taken. Each patient was given <br />medical treatment with tamoxifen (20 mg/day) for three months. SFA, FSH, <br />and testosterone were taken after three months. There was a significant <br />increase in FSH, testosterone, sperm concentration, and progressive motility <br />after treatment (p value &lt;0.001), furthermore, there was no significant <br />correlation between hormonal changes and changes in SFA study markers<br />(p value &gt; 0,05). Improvements in hormone levels following tamoxifen <br />treatment for patients with idiopathic oligoasthenoteratospermia do not <br />correlate with alterations in SFA markers.</p> Ali Abdulkhaleq Ula AL-kawaz Copyright (c) 2020 Ali Abdulkhaleq, Ula AL-kawaz 2020-08-08 2020-08-08 10 2 1 13 10.28969/IJEIR.v10.i2.r1.20