In Vitro Studying the Effect of Adding Autologous Platelet Rich Plasma (PRP) to the Human Semen on the Sperm DNA Integrity

Authors

  • Dhafer Abbas Hamdan High Institute of Infertility Diagnosis and Assisted Reproductive Technologies, Al Nahrain University, Baghdad, Iraq.
  • Ali Ibrahim Rahim University of Kufa, Najaf, Iraq https://orcid.org/0000-0003-4409-2050
  • Ula M. R. Al-Kawaz High Institute of Infertility Diagnosis and Assisted Reproductive Technologies, Al Nahrain University, Baghdad, Iraq. https://orcid.org/0000-0003-3998-2665

DOI:

https://doi.org/10.28969/IJEIR.v11.i2.r7

Keywords:

PRP, DNA, Sperm preparation, DFI, Sperm DNA fragmentation

Abstract

For conception and the development of healthy embryos, sperm DNA integrity is crucial. According to a growing body of studies, there is a strong correlation between sperm DNA damage and male infertility. Among the new medicines being developed in the medical field, the application of Platelet Rich Plasma (PRP) in human reproduction has yet to be examined. A total of 100 semen samples were used in the current experimental investigation. From November 2020 to June 2021, the research was conducted at the High Institute for Infertility Diagnosis and Assisted Reproductive Technologies. Masturbation was used to get an ejaculated semen sample. After semen analysis, the samples were separated into two equal parts, one without autologous PRP and the other with 2% autologous PRP, with the DNA fragmentation assessed using the Sperm Chromatin Dispersion Test. There was highly significant reduction in DNA fragmentation index (p < 0.001). The mean sperm DNA integrity was reduced after adding PRP (33.85±16.73 vs 38.55±16.64), Mean (± SE). PRP has been shown to improve human sperm DNA integrity.

Author Biographies

Dhafer Abbas Hamdan, High Institute of Infertility Diagnosis and Assisted Reproductive Technologies, Al Nahrain University, Baghdad, Iraq.

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He received his B.Sc. in Biology from Al-Mustansiriya University, Baghdad, Iraq in 2004. He has been an employer in the Iraqi health ministry, Al-Risafa health directorate, Baghdad, Iraq for 20 years. Recently, he graduated from the High Institute of Infertility Diagnosis and Assisted Reproductive Technologies, Al Nahrain University with a master's degree in Applied Embryology.

Ali Ibrahim Rahim, University of Kufa, Najaf, Iraq

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He received his M.B.Ch.B. 2005, Faculty of Medicine, University of Kufa. His M.Sc. in Applied Embryology 2011 and his Ph.D. in Infertility and Clinical Reproduction 2019, High Institute for Infertility Diagnosis and Assisted Reproductive Technologies, Al Nahrain University. He had a research fellowship in clinical embryology and ARTs 2010, at Lubeck Medical University, Germany (as a part of an M.Sc. study). He is a faculty member for primary and high studies in the Faculty of Medicine, University of Kufa. He is a faculty member in the College of Medicine, University of Al-Ameed. He is a faculty member for high studies in High Institute for Infertility Diagnosis and Assisted Reproductive Technologies, Al-Nahrain University. He is a trainer in the Arab and Iraqi Board for Medical Specialties in sub-specialty for Infertility and Assisted Reproductive Technologies. He is an administration member in the Iraqi Fertility Society. He is a trainer and consultant in many IVF centers in Iraq. He is a trainer and consultant in the IVF Center in Al-Kafeel Specialized Hospital. He has 10 years' experience as a senior clinical embryologist in the Fertility Center, Al-Sadr Medical City, Najaf. He was trained in many IVF Centers in Germany, Belgium, Iran and Jordan. He published a lot of studies in many national and international scientific journals.  

Ula M. R. Al-Kawaz, High Institute of Infertility Diagnosis and Assisted Reproductive Technologies, Al Nahrain University, Baghdad, Iraq.

mceclip2-defde7d6a7782797ca7be662593a78c3.png     He is a Professor of Urology at Al Nahrain University. He is a fellow of the Iraqi Board for Medical Specialization, Council of Urology since 2006, and a fellow of the European Board of Urology, since 2009. He published more than 27 articles both local and international. He supervised many M.Sc. and Ph.D. students.

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Published

2021-11-09

How to Cite

1.
Hamdan D, Rahim A, Al-Kawaz U. In Vitro Studying the Effect of Adding Autologous Platelet Rich Plasma (PRP) to the Human Semen on the Sperm DNA Integrity. IJEIR [Internet]. 2021 Nov. 9 [cited 2024 Dec. 8];11(2):90-100. Available from: https://ijeir.net/index.php/ijeir/article/view/v10i2r7_2021_ijeir

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Research Articles

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