In Vitro Studying the Effect of Adding Autologous Platelet Rich Plasma (PRP) to the Human Semen on the Sperm DNA Integrity
DOI:
https://doi.org/10.28969/IJEIR.v11.i2.r7Keywords:
PRP, DNA, Sperm preparation, DFI, Sperm DNA fragmentationAbstract
For conception and the development of healthy embryos, sperm DNA integrity is crucial. According to a growing body of studies, there is a strong correlation between sperm DNA damage and male infertility. Among the new medicines being developed in the medical field, the application of Platelet Rich Plasma (PRP) in human reproduction has yet to be examined. A total of 100 semen samples were used in the current experimental investigation. From November 2020 to June 2021, the research was conducted at the High Institute for Infertility Diagnosis and Assisted Reproductive Technologies. Masturbation was used to get an ejaculated semen sample. After semen analysis, the samples were separated into two equal parts, one without autologous PRP and the other with 2% autologous PRP, with the DNA fragmentation assessed using the Sperm Chromatin Dispersion Test. There was highly significant reduction in DNA fragmentation index (p < 0.001). The mean sperm DNA integrity was reduced after adding PRP (33.85±16.73 vs 38.55±16.64), Mean (± SE). PRP has been shown to improve human sperm DNA integrity.
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Author Biographies
Dhafer Abbas Hamdan, High Institute of Infertility Diagnosis and Assisted Reproductive Technologies, Al Nahrain University, Baghdad, Iraq.
He received his B.Sc. in Biology from Al-Mustansiriya University, Baghdad, Iraq in 2004. He has been an employer in the Iraqi health ministry, Al-Risafa health directorate, Baghdad, Iraq for 20 years. Recently, he graduated from the High Institute of Infertility Diagnosis and Assisted Reproductive Technologies, Al Nahrain University with a master's degree in Applied Embryology.
Ali Ibrahim Rahim, University of Kufa, Najaf, Iraq
He received his M.B.Ch.B. 2005, Faculty of Medicine, University of Kufa. His M.Sc. in Applied Embryology 2011 and his Ph.D. in Infertility and Clinical Reproduction 2019, High Institute for Infertility Diagnosis and Assisted Reproductive Technologies, Al Nahrain University. He had a research fellowship in clinical embryology and ARTs 2010, at Lubeck Medical University, Germany (as a part of an M.Sc. study). He is a faculty member for primary and high studies in the Faculty of Medicine, University of Kufa. He is a faculty member in the College of Medicine, University of Al-Ameed. He is a faculty member for high studies in High Institute for Infertility Diagnosis and Assisted Reproductive Technologies, Al-Nahrain University. He is a trainer in the Arab and Iraqi Board for Medical Specialties in sub-specialty for Infertility and Assisted Reproductive Technologies. He is an administration member in the Iraqi Fertility Society. He is a trainer and consultant in many IVF centers in Iraq. He is a trainer and consultant in the IVF Center in Al-Kafeel Specialized Hospital. He has 10 years' experience as a senior clinical embryologist in the Fertility Center, Al-Sadr Medical City, Najaf. He was trained in many IVF Centers in Germany, Belgium, Iran and Jordan. He published a lot of studies in many national and international scientific journals.
Ula M. R. Al-Kawaz, High Institute of Infertility Diagnosis and Assisted Reproductive Technologies, Al Nahrain University, Baghdad, Iraq.
He is a Professor of Urology at Al Nahrain University. He is a fellow of the Iraqi Board for Medical Specialization, Council of Urology since 2006, and a fellow of the European Board of Urology, since 2009. He published more than 27 articles both local and international. He supervised many M.Sc. and Ph.D. students.
References
Zegers-Hochschild F, Adamson GD, Dyer S, Racowsky C, de Mouzon J, Sokol R, et al. The International Glossary on Infertility and Fertility Care, 2017†‡§. Human Reproduction. Oxford University Press (OUP); 2017 Jul 28;32(9):1786–801. [Online article link]
Choy JT, Eisenberg ML. Male infertility as a window to health. Fertility and Sterility. Elsevier BV; 2018 Oct;110(5):810–4. [Online article link]
Wang C, Swerdloff RS. Limitations of semen analysis as a test of male fertility and anticipated needs from newer tests. Fertility and Sterility. Elsevier BV; 2014 Dec;102(6):1502–7. [Online article link]
Agarwal A, Allamaneni S. Sperm DNA damage assessment: a test whose time has come. Fertility and Sterility. Elsevier BV; 2005 Oct;84(4):850–3. [Online article link]
Guzick DS, Overstreet JW, Factor-Litvak P, Brazil CK, Nakajima ST, Coutifaris C, et al. Sperm Morphology, Motility, and Concentration in Fertile and Infertile Men. New England Journal of Medicine. Massachusetts Medical Society; 2001 Nov 8;345(19):1388–93. [Online article link]
Simon L, Emery BR, Carrell DT. Review: Diagnosis and impact of sperm DNA alterations in assisted reproduction. Best Practice & Research Clinical Obstetrics & Gynaecology. Elsevier BV; 2017 Oct;44:38–56. [Online article link]
Carmona J, Argüelles D, Prades M. Transforming growth factor beta-3 and nitric oxide levels in four autologous platelet concentrates and plasma derived from equine blood. Archivos de Medicina Veterinaria, 2008;40(2):155-160. [Online article link]
Anitua E, Andia I, Ardanza B, Nurden P, Nurden A. Autologous platelets as a source of proteins for healing and tissue regeneration. Thrombosis and Haemostasis. Georg Thieme Verlag KG; 2004;91(01):4–15. [Online article link]
Rodríguez J, Palomar M, García J. Platelet-rich plasma: biological foundations and applications in maxillofacial surgery and facial aesthetics/platelet-rich plasma: biology and applications in maxillofacial surgery and facial aesthetics. Revista Espanola de Cirugia Oral y Maxilofacial. 2012;34(1):8-17. [Online article link]
Lubkowska A, Dolegowska B, Banfi G. Growth factor content in PRP and their applicability in medicine. Journal of Biological Regulators & Homeostatic Agents. 2012 Apr-Jun;26(2 Suppl 1):3S-22S [Online article link]
Magalon J, Bausset O, Serratrice N, Giraudo L, Aboudou H, Veran J, et al. Characterization and Comparison of 5 Platelet-Rich Plasma Preparations in a Single-Donor Model. Arthroscopy: The Journal of Arthroscopic & Related Surgery. Elsevier BV; 2014 May;30(5):629–38. [Online article link]
Bader R, Ibrahim JN, Moussa M, Mourad A, Azoury J, Azoury J, et al. In vitro effect of autologous platelet‐rich plasma on H 2 O 2 ‐induced oxidative stress in human spermatozoa. Andrology. Wiley; 2019 May 12;8(1):191–200. [Online article link]
Agarwal A, Sharma R, Ahmad G. Sperm chromatin assessment. In Textbook of Assisted Reproductive Techniques. 2017, 107-129. CRC Press. [Online article link]
Perumal P. Effect of Superoxide Dismutase on Semen Parameters and Antioxidant Enzyme Activities of Liquid Stored (5°C) Mithun (Bos frontalis) Semen. Journal of Animals. Hindawi Limited; 2014 Mar 13;2014:1–9. [Online article link]
Lee HS, Park Y-S, Lee JS, Seo JT. Serum and seminal plasma insulin-like growth factor-1 in male infertility. Clinical and Experimental Reproductive Medicine. The Korean Society for Reproductive Medicine; 2016;43(2):97. [Online article link]
Zhao J, Dong X, Hu X, Long Z, Wang L, Liu Q, et al. Zinc levels in seminal plasma and their correlation with male infertility: A systematic review and meta-analysis. Scientific Reports. Springer Science and Business Media LLC; 2016 Mar;6(1). [Online article link]
Susilowati S, Triana IN, Malik A. The effects of insulin-like growth factor I (IGF-I) complex from seminal plasma on capacitation, membrane integrity and DNA fragmentation in goat spermatozoa. Asian Pacific Journal of Reproduction. Asian Pacific Journal of Tropical Medicine Press; 2015 Sep;4(3):208–11. [Online article link]
Obermair A, Obruca A, Pöhl M, Kaider A, Vales A, Leodolter S, et al. Vascular endothelial growth factor and its receptors in male fertility. Fertility and Sterility. Elsevier BV; 1999 Aug;72(2):269–75. [Online article link]
Di Leo V. Effect of zinc supplementation on trace elements and intestinal metallothionein concentrations in experimental colitis in the rat. Digestive and Liver Disease. Elsevier BV; 2001 Mar;33(2):135–9. [Online article link]
Suriya J, Bharathiraja S, Sekar V, Rajasekaran R. Metallothionein induction and antioxidative responses in the estuarine polychaeta Capitella capitata (Capitellidae). Asian Pacific Journal of Tropical Biomedicine. Medknow; 2012 Feb;2(2):S1052–S1059. [Online article link]
Björndahl L, Kvist U. A model for the importance of zinc in the dynamics of human sperm chromatin stabilization after ejaculation in relation to sperm DNA vulnerability. Systems Biology in Reproductive Medicine. Informa UK Limited; 2011 Jan;57(1-2):86–92. [Online article link]
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