Correlation of serum and follicular fluid EMMPRIN levels at day of ova pick up and embryo transfer to endometrial thickness and its effect on ICSI outcomes in fresh cycles


  • Methaq Almousa high institute of infertility diagnosis and assisted reproductive technologies,ALNahrain University
  • Amal Abdulwahid Mohammed The High Institute for Infertility Diagnosis and Assisted Reproductive Technologies
  • Ali Ibrahim Rahim Faculty of Medicine, University of Kufa, Najaf, Iraq



EMMPRIN, Basigin, Serum, Follicular fluid, ICSI


EMMPRIN (Basigin) is a potent inducer of matrix metalloproteinases
(MMPs). Expression of EMMPRIN in the uterus has a very important
role in implantation of embryo, this study was conducted to evaluate the
relationship of pregnancy rates with EMMPRIN levels in follicular fluid
and serum of intracytoplasmic sperm injection patients. The study was
cross sectional study analyzed the serum and follicular fluid samples of
58 patients undergo ICSI procedure. The serum and follicular fluid
samples were collected from infertile women aged 20 to 42 years.
However, the levels of EMMPRIN were measured in the serum and
follicular fluid samples and these samples were obtained during oocytes
retrieval and another serum samples were collected at the day of embryo
transfer. All women have ovarian stimulation with recombinant follicle
stimulating hormone (FSH) at the second or third day of the cycle.
Result: The pregnancy rate was 27.6 %. The present study showed that
EMMPRIN in serum and follicular fluid at day of ova pickup and its level
at embryo transfer day, are significantly low in pregnant women (p <
0.05) compared to non-pregnant women. The statistical analysis showed
no significant correlation between serum and follicular fluid EMMPRIN
with endometrial thickness (p > 0.05).
EMMPRIN level can be used as a promising parameter for the prediction
of pregnancy rate in ICSI cycles


Benkhalifa, M., Zayani, Y., Bach, V., Copin, H., Feki, M., Benkhalifa, M., & Allal-Elasmi, M. (2018). Does the dysregulation of matrix metalloproteinases contribute to recurrent implantation failure?. Expert Review of Proteomics, 15(4), 311-323.‏

Braundmeier AG, Fazleabas AT, Lessey BA, Guo H, Toole BP & Nowak RA 2006 Extracellular matrix metalloproteinase inducer regulates metalloproteinases in human uterine endometrium. Journal of Clinical Endocrinology & Metabolism 2358–2365.ce. Reproduction 1057–1066.

Chen L, Bi J, Nakai M, Bunick D, Couse JF, Korach KS & Nowak RA 2010 Expression of basigin in reproductive tissues of estrogen receptor-?? or -?? null mice. Reproduction 1057–1066.

Dang, Y., Li, W., Tran, V. and Khalil, R.A., 2013. EMMPRIN-mediated induction of uterine and vascular matrix metalloproteinases during pregnancy and in response to estrogen and progesterone. Biochemical pharmacology, 86(6), pp.734-747.

Davis, J. S., & LaVoie, H. A. (2019). Molecular regulation of progesterone production in the corpus luteum. In The ovary (pp. 237-253). Academic Press.‏

Esteves, S. C., Roque, M., Bedoschi, G., Haahr, T., & Humaidan, P. (2018). Intracytoplasmic sperm injection for male infertility and consequences for offspring. Nature Reviews Urology, 15(9), 535-562.‏

Fournier, S. B., D’Errico, J. N., & Stapleton, P. A. (2021). Uterine Vascular Control Preconception and During Pregnancy. Comprehensive Physiology, 11(3), 1871.‏

Governini, L., Luongo, F. P., Haxhiu, A., Piomboni, P., & Luddi, A. (2021). Main actors behind the endometrial receptivity and successful implantation. Tissue and Cell, 73, 101656.‏

Griesinger, G., Blockeel, C., Kahler, E., Pexman-Fieth, C., Olofsson, J. I., Driessen, S., & Tournaye, H. (2020). Dydrogesterone as an oral alternative to vaginal progesterone for IVF luteal phase support: A systematic review and individual participant data meta-analysis. PloS one, 15(11), e0241044.‏.;

Li, K., & Nowak, R. A. (2020). The role of basigin in reproduction. Reproduction, 159(2), R97-R109.‏

Noguchi Y, Sato T, Hirata M, Hara T, Ohama K & Ito A 2003 Identification and characterization of extracellular matrix metalloproteinase inducer in human endometrium during the menstrual cycle in vivo and in vitro. Journal of Clinical Endocrinology & Metabolism 6063–6072.

Scolari, S. C., Pugliesi, G., Strefezzi, R. D. F., Andrade, S. C., Coutinho, L. L., & Binelli, M. (2017). Dynamic remodeling of endometrial extracellular matrix regulates embryo receptivity in cattle. Reproduction, 153(1), 49-61.‏

Takmaz, O., Yozgatli, D., Ozaltin, S., Ozbasli, E., Kocyigit, Y., Kuran, S. B., ... & Bastu, E. (2019). Can follicular Emmprin and BMP 4 levels predict ICSI outcome?. Journal of assisted reproduction and genetics, 36(6), 1127-1133.‏

Yoshii, N., Hamatani, T., Inagaki, N., Hosaka, T., Inoue, O., Yamada, M.,Machiya, R., Yoshimura, Y. and Odawara, Y., 2013. Successful implantation after reducing matrix metalloproteinase activity in the uterine cavity. Reproductive Biology and Endocrinology, 11(1), pp.1-10.



How to Cite

Almousa M, Abdulwahid Mohammed A, Ibrahim Rahim A. Correlation of serum and follicular fluid EMMPRIN levels at day of ova pick up and embryo transfer to endometrial thickness and its effect on ICSI outcomes in fresh cycles. IJEIR [Internet]. 2022 Jun. 8 [cited 2024 Jun. 18];12(1):16-25. Available from: