the effect of in vitro exposure to certain metformin doses on the motility of human spermatozoa


  • Fatima Emad Al-Nashme The High Institute for Infertility Diagnosis and Assisted Reproductive Technologies
  • Wasan Adnan Al-Jubory The High Institute for Infertility Diagnosis and Assisted Reproductive Technologies



metformin, spermatozoa, motility, AMPK


Metformin is an anti-hyperglycemic medication used to treat type 2
diabetes mellitus. Regardless of its anti-diabetic effect, metformin has a
non-genomic action and it could be a beneficial additive to treat the sperm
which considered as transcriptionally dormant cell and also a molecule
that could rapidly adjust the metabolism of spermatozoa to adapt it to the
surrounding environment. Sixty-four normozoospermic semen sample
included in the study. Each sample incubated for 30 minutes with
metformin (2.5 and 0.5 µM) and SFA was conducted to each sample.
There was a significant decrease in total motility (progressive and nonprogressive)

in samples incubated with both concentrations compared
with the neat samples. While the samples incubated with 0.5 µM shows
a lower motility compared with 2.5 µM. Metformin significantly reduced
total motility of fresh spermatozoa at both concentrations.


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How to Cite

Emad Al-Nashme F, Adnan Al-Jubory W. the effect of in vitro exposure to certain metformin doses on the motility of human spermatozoa. IJEIR [Internet]. 2022 Jun. 12 [cited 2023 Nov. 29];12(1):55-67. Available from:



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