DFI and Chromatin Maturity Status Could be Considered as Independent Parameters for Male Fertility Assessment

Authors

  • Huda Abdalrazaq High Institute of Infertility Diagnosis and Assisted Reproductive Technologies, Al Nahrain University, Baghdad, Iraq
  • Ali Ibrahim Rahim 2 Al-Ameed University, College of Medicine; Al-Kafeel Specialized Hospital, IVF Center; Karbala https://orcid.org/0000-0003-4409-2050
  • Laith Amer Abd AL- Hussein College of Medicine, Al Nahrain University, Baghdad, Iraq

DOI:

https://doi.org/10.28969/IJEIR.v10.i2.r7.20

Keywords:

DNA, Sperm Chromatin, Assisted Reproductive Technologies (ART), DNA Fragmentation Index (DFI)

Abstract

Human sperm chromatin and integrity of DNA have multiple endogenous factors, such as sperm compression and nucleoproteins, with various mechanisms all through their sperm production and transport. The integrity of the paternal genetic material in both regular and assisted pregnancy is essential for initiating and maintaining a viable pregnancy. This study included semen samples from 100 men taken from Al Nahrain University Medical Clinic and Babylon Teaching Hospital for Women and Children. The first sperm test was based on the World Health Organization (1999), followed by a DNA integrity test that uses a DNA fragmentation test (DFI), and the third intensification test Chromatin by aniline blue dye. It was correlated negatively with the sperm count in combination with DFI and chromatin sperm with semen characteristics (r = - 0.223, p = 0.026). As well as DFI, sperm chromatin was being clinically significant associated with the rate of round cells (r = 0.39, < 0.001). DFI was positive and significantly associated with sperm chromatin in all infertile men (r = 0.958; p<0.001). DNA isolation in all enrolled men has been adversely linked with sperm/ejaculation, has been associated with the round cell count positive way and is not understanding and resolving with other parameters. The width of abstinence and beneficial cell count in all register men were strongly linked with Sperm chromatin. There was a direct relationship between the fragmentation of DNA and chromatin. This could imply that one exam can be used instead of the other.

Author Biographies

Ali Ibrahim Rahim, 2 Al-Ameed University, College of Medicine; Al-Kafeel Specialized Hospital, IVF Center; Karbala

He received his M.B.Ch.B. 2005, Faculty of Medicine, University of Kufa. His M.Sc. in Applied Embryology 2011 and his Ph.D. in Infertility and Clinical Reproduction 2019, High Institute for Infertility Diagnosis and Assisted Reproductive Technologies, Al Nahrain University. He had a research fellowship in clinical embryology and ARTs 2010, at Lubeck Medical University, Germany (as a part of an M.Sc. study). He is a Faculty member for primary and high studies in the Faculty of Medicine, University of Kufa. He is a Faculty member in the College of Medicin, University of Al-Ameed. He is a faculty member for high studies in High Institute for Infertility Diagnosis and Assisted Reproductive Technologies, Al-Nahrain University. He is a trainer in the Arab and Iraqi Board for Medical Specialties in sub-specialty for Infertility and Assisted Reproductive Technologies. He is an administratiion member in the Iraqi Fertility Society. He is a trainer and consultant in many IVF centers in Iraq. He is a trainer and consultant in the IVF Center in Al-Kafeel Specialized Hospital. He has 10 years' experience as a senior clinical embryologist in the Fertility Center, Al-Sadr Medical City, Najaf. He was trained in many IVF Centers in Germany, Belgium, Iran and Jordan. He published a lot of studies in many national and international scientific journals.

Laith Amer Abd AL- Hussein, College of Medicine, Al Nahrain University, Baghdad, Iraq

He is a Urology and Nephrology Specialist with an Arab Board and Iraqi Board.
He is a Lecturer at Al Nahrain University, College of Medicine.

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Published

2020-08-08

How to Cite

1.
Abdalrazaq H, Rahim A, Abd AL- Hussein L. DFI and Chromatin Maturity Status Could be Considered as Independent Parameters for Male Fertility Assessment. IJEIR [Internet]. 2020 Aug. 8 [cited 2024 Apr. 20];10(2):107-15. Available from: https://ijeir.net/index.php/ijeir/article/view/44

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