Mitofusin 1 as Marker of Oocyte Maturation in Relevance to ICSI Outcome in Infertile Females
DOI:
https://doi.org/10.28969/IJEIR.v13.i2.r4.23Keywords:
mitofusin-1 , ICSI, pregnancy, infertility, IraqAbstract
Pro-fusion proteins as mitofusins-1 are required for controlling
mitochondrial shape which determined by a dynamic balance between
organelle fusion and fission and also supports oocyte development. When
compare with somatic cell, mitochondria of oocyte are tiny and circular in
presence. The aim is to study the mitofusin-1 in the follicular fluid as a marker
for evaluating of oocyte maturation in women undergoing ICSI cycles. Fifty
infertile females were included in cross-section study was undergoing ICSI
procedure with age 20 to 42 years. After retrieval of oocyte and the number
of oocytes was recorded by embryologist and follicular fluid Samples were
used for the measurement of Mitofusin 1. Mitofusin 1 levels by ELISA kit
(Mybiosource /USA).The results showed considerable higher mitofusin1levels in patients with good oocytes quality (3.71 ± 1.35 vs. 2.45 ± 1.12 &
p=0.001). There were much positive correlations between follicular fluids
mitofusin-1 with both total oocytes count (r= 0.374 & p= 0.007) and MII
oocytes (r=0.383 & p=0.006); and there was no much correlation between
mitofusin-1 levels with MI oocyte (r= - 0.100 & p= 0.490), germinal vesical
oocyte (r=0.103 & p= 0.475) and fertilization rate (r= 0.054 & p= 0.711). High
follicular fluid levels of Mitofusin 1 may positively impact oocyte
development and pregnancy rate.
Downloads
References
Abebe, M. S., Afework, M., & Abaynew, Y. (2020). Primary and secondary infertility in Africa: systematic review with meta-analysis. Fertility Research and Practice, 6(1), 1-11. https://doi.org/10.1186/s40738-020-00090-3
Babayev, E. & Seli,E. (2015) Oocyte mitochondrial function and reproduction. Curr. Opin. Obstet. Gynecol. 27, 175–181. DOI: 10.1097/GCO.0000000000000164
BUISMAN, E. T., GRENS, H., WANG, R., BHATTACHARYA, S., BRAAT, D., HUPPELSCHOTEN, A. & VAN DER STEEG, J. W. (2022). Trends in research on pain relief during oocyte retrieval for IVF/ICSI: a systematic, methodological review. Human Reproduction Open, 2022, hoac006 DOI: 10.1093/hropen/hoac006
CHOPRA, M., BUDHWAR, M., SHARMA, M. & SOBTI, R. C. (2023). In Vitro Fertilization and Embryo Transfer. Biodiversity. CRC Press https://www.taylorfrancis.com/chapters/edit/10.1201/9781003220398-20/vitro-fertilization-embryo-transfer-mani-chopra-muskan-budhwar-madhu-sharma-ranbir-chandra-sobti
Orazov, M. R., Radzinsky, V. Y., Ivanov, I. I., Khamoshina, M. B., & Shustova, V. B. (2019). Oocyte quality in women with infertility associated endometriosis. Gynecological Endocrinology, 35(sup1), 24- 26. DOI: 10.1080/09513590.2019.1632088
Rienzi, L, Balaban, B, Ebner, T & Mandelbaum, J 2012, Oocyte retrieval and selectionʼ, In Textbook of assisted reproductive techniques ,pp. 136-53. CRC Press.ʼ, doi:10.1093/hum rep/des200.
Scanlan, J. L. Robin, C. & Mirth, C. 2022. Rethinking The Ecdysteroid Source During Drosophila Pupal–Adult Development. Insect Biochemistry And Molecular Biology, 103891. DOI: 10.1016/j.ibmb.2022.103891
Udagwa, O., ISHIHARA, T., MAEDA, M., MA
, Y., TSUKAMOTO, S., KAWANO, N
MIYADO, K., SHITARA, H., YOKOTA, S. & NOMURA, M. (2014). Mitochondrial fission factor Drp1 maintains oocyte quality via dynamic rearrangement of multiple organelles. Current Biology, 24, 2451- 2458. DOI: 10.1016/j.cub.2014.08.060
Vander Borght, M., & Wyns, C. (2018). Fertility and infertility: Definition and epidemiology. Clinical biochemistry, 62, 2-10. DOI: 10.1016/j.clinbiochem.2018.03.012
ZHAO, S., HENG, N., WANG, H., WANG, H., ZHANG, H., GONG, J., HU, Z. & ZHU, H. (2022). Mitofusins: from mitochondria to fertility. Cellular and Molecular Life Sciences, 79, 370. doi: 10.1007/s00018-022-04386-z
ZHENG, D., ZENG, L., YANG, R., LIAN, Y., ZHU, Y.-M., LIANG, X., TANG, L., WANG, H., CAO, Y. & HAO, G. (2019). Intracytoplasmic sperm injection (ICSI) versus conventional in vitro fertilisation (IVF) in couples with nonsevere male infertility (NSMI-ICSI): protocol for a multicentre randomised controlled trial. BMJ open, 9, e030366. DOI: 10.1136/bmjopen-2019-030366
Downloads
Published
How to Cite
Issue
Section
Categories
License
Copyright (c) 2023 Rana riyadh khalil tememe, Mufeda AL-Ammar, Hayder A. L. Mossa
This work is licensed under a Creative Commons Attribution 4.0 International License.
