Mitofusin 1 as Marker of Oocyte Maturation in Relevance to ICSI Outcome in Infertile Females

Authors

  • Rana R. Khalil The High Institute for Infertility Diagnosis and Assisted Reproductive Technologies
  • Mufeda AL-Ammar The High Institute for Infertility Diagnosis and Assisted Reproductive Technologies
  • Hayder A. L. Mossa The High Institute for Infertility Diagnosis and Assisted Reproductive Technologies

DOI:

https://doi.org/10.28969/IJEIR.v13.i2.r4.23

Keywords:

mitofusin-1 , ICSI, pregnancy, infertility, Iraq

Abstract

Pro-fusion proteins as mitofusins-1 are required for controlling
mitochondrial shape which determined by a dynamic balance between
organelle fusion and fission and also supports oocyte development. When
compare with somatic cell, mitochondria of oocyte are tiny and circular in
presence. The aim is to study the mitofusin-1 in the follicular fluid as a marker
for evaluating of oocyte maturation in women undergoing ICSI cycles. Fifty
infertile females were included in cross-section study was undergoing ICSI
procedure with age 20 to 42 years. After retrieval of oocyte and the number
of oocytes was recorded by embryologist and follicular fluid Samples were
used for the measurement of Mitofusin 1. Mitofusin 1 levels by ELISA kit
(Mybiosource /USA).The results showed considerable higher mitofusin1levels in patients with good oocytes quality (3.71 ± 1.35 vs. 2.45 ± 1.12 &
p=0.001). There were much positive correlations between follicular fluids
mitofusin-1 with both total oocytes count (r= 0.374 & p= 0.007) and MII
oocytes (r=0.383 & p=0.006); and there was no much correlation between
mitofusin-1 levels with MI oocyte (r= - 0.100 & p= 0.490), germinal vesical
oocyte (r=0.103 & p= 0.475) and fertilization rate (r= 0.054 & p= 0.711). High
follicular fluid levels of Mitofusin 1 may positively impact oocyte
development and pregnancy rate.

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Published

2023-11-08

How to Cite

1.
Khalil RR, AL-Ammar M, A. L. Mossa H. Mitofusin 1 as Marker of Oocyte Maturation in Relevance to ICSI Outcome in Infertile Females. IJEIR [Internet]. 2023 Nov. 8 [cited 2024 Apr. 20];13(2):39-50. Available from: https://ijeir.net/index.php/ijeir/article/view/158