The Effect of Smoking on the Apoptotic Marker (Caspase-3) of Human Sperm
DOI:
https://doi.org/10.28969/IJEIR.v14.i1.r7.24Keywords:
Smoking, Apoptosis, HOST, Caspase-3Abstract
Background: The ability of human sperm to fertilize can be compromised both in vivo and in vitro by the toxins found in cigarette smoke, which can lower sperm mitochondrial activity and harm chromatin structure.
Aim of the study: to study the effect of smoking on the apoptotic marker (caspase-3) of human sperm.
Patients, materials and methods: A cross-sectional study of 80 semen sample of sub-fertile men, they were divided into smokers (35 patients) and non-smokers (45 patients). All the semen samples after seminal fluid analysis underwent HOST test and grading (A-G) according to the WHO 2021 criteria then the slides smeared and stained with immunocytochemistry kit for Caspase-3 apoptotic marker.
Results: There was no significant effect of smoking in the concentration of caspase-3 expression, in spite of high presentation of both grades A and G in smoker group (p = 0.57, 0.56 respectively).
Conclusion: Smoking has a negative impact on the DNA integrity of sperm as it increases the apoptotic marker within the smoker group.
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Copyright (c) 2024 Mohanad Mohammed, Muayad Saribet, Rehab Sh. Al-Maliki
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