Association of methylene tetrahydrofolate reductase polymorphisms with cycle day two hormonal characteristics


  • Rabab Al-yasiry Department of Anatomy, and Histology, College of Medicine / Babylon University, Babylon / Iraq
  • Mufeda AL-Ammar The High Institute for Infertility Diagnosis and Assisted Reproductive Technologies
  • Muayad S. Abbood The High Institute for Infertility Diagnosis and Assisted Reproductive Technologies



The 5,10-methylenetetrahydrofolate reductase (MTHFR) enzyme is a key
methionine and folate metabolism enzyme that is found in oocytes and
embryos in preimplantation stages and plays a vital role in female
reproduction.To assess the impact of MTHFR genetic polymorphisms
(C677T and A1298C) on follicular stimulating hormone (FSH), luteinizing
hormone(LH), Estradiol (E2), progesterone and antimullerian hormone
(AMH) in female receiving intracytoplasmic sperm injection (ICSI), 85
infertile women undergoing ICSI treatment at the High Institute of Infertility
Diagnosis and Assisted Reproductive Technologies in Baghdad, Iraq, were
recruited. These patients had their MTHFR polymorphism genotypes
examined, FSH, LH, E2, progesterone, and AMH levels were compared
between groups. Furthermore, all demographic characteristics as well as ICSI
outcomes were compared between pregnant and non-pregnant women. The
research revealed no discernible variation between the means of FSH, LH, E2
and AMH with respect to the 219A>C(rs1801131) (A1298C) genotypes (p >
0.05). Progesterone level was significantly differed with high level found in
CC followed by AA and AC. There was no statistically significant variation
in hormonal features between CC, CT, and TT for A1298C,
219A>C(rs1801133). The demographic features (age, duration, type of
infertility, cause of infertility and previous trial) were not notably different
between women who were pregnant and those who were not. In women
undergoing ICSI, the maternal MTHFR polymorphisms is not related to FSH,
LH, E2 and AMH. MTHFR polymorphisms therefore don't offer useful
information regarding the hormonal levels in our groups


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How to Cite

Al-yasiry R, AL-Ammar M, S. Abbood M. Association of methylene tetrahydrofolate reductase polymorphisms with cycle day two hormonal characteristics. IJEIR [Internet]. 2023 Nov. 8 [cited 2024 Apr. 20];13(2):78-9. Available from:

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