Phosphatidylserine Levels in Seminal Plasma of Iraqi Infertile Men Using Direct and Indirect Swim Up Sperm Preparation Techniques
DOI:
https://doi.org/10.28969/IJEIR.v13.i2.r5.23Keywords:
Key Words: PhosphatidylSerine (PS), Direct Swim Up (DSU),Indirect Swim Up (IDSU),Infertile men.Abstract
Phosphatidylserine (PS), exposed on viable sperm, is required for
fertilization as it is recognized by specific receptors located on the microvilli
of the oocyte to promote sperm: egg fusion. The heads of viable and motile
sperm exhibit PS. With (PS) exposure progressively increasing during sperm
transit through the epididymis. PS asymmetry on the leaflets of plasma
membrane is arranged in a complex way, via multiple proteins functioning in
opposite directions; further, PS is also essential for many intracellular
trafficking events. The aim of this study was to evaluate the
phosphatidylserine PS level in seminal plasma of infertile men before and
after activation using Direct Swim Up (DSU) and Indirect Swim Up (IDSU)
activation method and make a comparison between them. Forty infertile men
were included in this study during their attendance to the infertility clinic at
High Institute for Infertility Diagnosis and Assisted Reproductive
Technologies, Al-Nahrain University. Normozoospermic or mild
asthenozoospermic semen samples were used to choose the cases, according
to WHO manual standards (1999 -2010). The collected semen samples
obtained were divided into three parts: The first part prepared was as an in
vitro sperm characterization before activation while the second and third parts
were after activation using direct and indirect swim up techniques
respectively. The level of seminal plasma phosphatidylserine was increased
after the activation techniques from the baseline level before activation.
Comparison of Seminal plasma phosphatidylserine levels between direct and
indirect sperm activation technique were significantly higher in direct swim
up activation technique than indirect one. Phosphatidylserine (PS) in semen
plasma of infertile men was significantly increased after activation using
Direct Swim Up DSU preparation method.
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Copyright (c) 2023 Dhameaa A. Neamah, Hayder A. L. Mossa, Muayad S. Abbood
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