Leukemia Inhibitory Factor as an Indicator for Determination of the Optimal Time of Intrauterine PRP Infusion In ICSI Cases

Sahar S. Raheem; Mufeeda Alammar; Muaed Sarbeet

doi:10.28969/IJEIR.v13.i2.r1.23

Authors

Sahar S. Raheem College of Medicine, Al-Muthanna University, AL-Muthana
Mufeeda Alammar The High Institute for Infertility Diagnosis and Assisted Reproductive Technologies https://orcid.org/0000-0002-7995-6755
Muaed Sarbeet The High Institute for Infertility Diagnosis and Assisted Reproductive Technologies https://orcid.org/0000-0002-0262-3813

DOI:

https://doi.org/10.28969/IJEIR.v13.i2.r1.23

Keywords:

PRP, ICSI, ET, RI, PI, LIF

Abstract

The compromise of uterine receptivity is one of the main reasons for the
failure of assisted reproduction techniques. Leukemia inhibitory factor is one
of those molecular parameters that allow us to identify an endometrium as
receptive. Platelet rich plasma (PRP) contains a range of cytokines and growth
factors including leukemia inhibitory factor that encourage the migration,
proliferation, and differentiation of cells. Treatments for antagonist ovarian
stimulation were administered to 75 women under the age of 40, and 2-D
power doppler ultrasound was used to guide the oocyte harvest. Patients were
allocated into three groups according to the time of intrauterine infusion of
PRP (no PRP, PRP at triggering day and PRP at ova pickup day). Power
Doppler is utilized to measure endometrial thickness (ET), pulsatility index
(PI), and resistance index (RI) of sub endometrial arteries, as well as serum
levels of leukemia inhibitory factor (LIF). The mean endometrial thickness as
well as serum level of LIF in PRP trigger group were higher, resistance index
and pulsatility index were lower in a significant manner than control and PRP
ova pickup group at all the days of study (triggering, ova pickup and embryo
transfer days). These findings revealed that intrauterine PRP infusion on ova
pick up day is associated with a significant increase in serum level of LIF that
in turn enhances endometrial thickness for patients scheduled for
intracytoplasmic sperm injection (ICSI).

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