The role of sperm DNA integrity in seminal plasma of infertile men
DOI:
https://doi.org/10.28969/IJEIR.v13.i1.r3.23Keywords:
Reactive oxygen species, DNA Fragmentation Index (DFI), Infertile men, DNA IntegrityAbstract
One-half of the genome is delivered by the sperm, and a healthy sperm is
needed for fertilization, fetal development, and child wellbeing. Abnormal
sperm DNA can impact any of these processes. The measurement of sperm
DNA fragmentation has been added to some medical andrology laboratories
as a crucial addition to the standard analysis of the semen. Sperm DNA
fragmentation has also been linked to male infertility including idiopathic
male infertility. Some degree of controlled DNA nicking is essential for
adequate DNA compaction, but excessive sperm DNA fragmentation is
usually associated with reduced male fertility potential, reduced fertilization,
poor embryo quality, recurrent pregnancy loss and poor assisted reproductive
techniques outcomes. Semen samples from 50 infertile males (as 19
asthenozoospermia, 7 oligozoospermia, 13 normospermia and 11 combined
defects) were analyzed for sperm DNA fragmentation. This study followed
the World Health Organization 2010 guidelines to assess the sperm
parameters. Sample were collected by masturbation and put in a 37 °C
incubator to be liquefied, then they were placed in a centrifuge (3000 rpm for
10 min) to extract the pellet, which was used for assessment of DFI% by
anillin blue staining. Negative significant correlation (p = 0.023) between
morphologically normal sperms and DFI % was found in normozoospermia
group. The objective of this study is to examine the correlation of DFI among
different seminal fluid categories (normozoospermia, oligozoospermia,
asthenozoospermia and, combined).
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References
Ali MK. Influence of Different Glutathione Concentrations on Human Sperm Cryopreservation. [Baghdad, Iraq]: Al-Nahrain University High Institute of Infertility Diagnosis and Assisted Reproductive Technologies; 2022.
Atig F, Kerkeni A, Saad A, Ajina M. Effects of reduced seminal enzymatic antioxidants on sperm DNA fragmentation and semen quality of Tunisian infertile men. J Assist Reprod Genet. 2017 Mar;34(3):373–81. https://doi.org/10.1007/s10815-013-9936-x
Bisconti M, Simon JF, Grassi S, Leroy B, Martinet B, Arcolia V, et al. Influence of Risk Factors for Male Infertility on Sperm Protein Composition. International Journal of Molecular Sciences. 2021 Jan;22(23):13164. https://doi.org/10.3390/ijms222313164
Brahem S, Mehdi M, Elghezal H, Saad A. Detection of DNA fragmentation and meiotic segregation in human with isolated teratozoospermia. J Assist Reprod Genet. 2011 Jan;28(1):41–8. https://doi.org/10.1007/s10815-010-9482-8
Choucair FB, Rachkidi EG, Raad GC, Saliba EM, Zeidan NS, Jounblat RA, et al. High level of DNA fragmentation in sperm of Lebanese infertile men using Sperm Chromatin Dispersion test. Middle East Fertility Society Journal. 2016;21(4):269–76. https://doi.org/10.1016/j.mefs.2016.06.005
Fadhil HA, Alali I, Hadi H. Human sperm DNA fragmentation and its correlation with antioxidant markers and sperm characteristics in Iraqi males. 2020 Jan 1;
Ferrigno A, Ruvolo G, Capra G, Serra N, Bosco L. Correlation between the DNA fragmentation index (DFI) and sperm morphology of infertile patients. J Assist Reprod Genet. 2021 Apr 1;38(4):979–86. https://doi.org/10.1007/s10815-021-02080-w
Hussein E. Seminal Plasma Coenzyme Q10 Level and Sperm DNA Integrity in Relevance to Sperm Parameters in Infertile Men. [High Institute for Infertility Diagnosis and Assisted Reproductive Technologies]: Al- Nahrain University; 2023.
Jebur RH. Effects of Pre-Freeze Addition of Melatonin on Human Sperm Cryo-tolerance. [Baghdad]: High Institute for Infertility Diagnosis and Assisted Reproductive Technologies; 2022.
Leach M, Aitken RJ, Sacks G. Sperm DNA fragmentation abnormalities in men from couples with a history of recurrent miscarriage. Australian and New Zealand Journal of Obstetrics and Gynaecology. 2015;55(4):379–83. https://doi.org/10.1111/ajo.12373
Magdum M, Chowdhury MAT, Begum N, Riya S. Types of Infertility and Its Risk Factors among Infertile Women: A Prospective Study in Dhaka City. Journal of Biosciences and Medicines. 2022 Mar 30;10(4):158–68. https://doi.org/10.4236/jbm.2022.104014
Pandruvada S, Royfman R, Shah TA, Sindhwani P, Dupree JM, Schon S, et al. Lack of trusted diagnostic tools for undetermined male infertility. J Assist Reprod Genet. 2021 Feb;38(2):265–76. https://doi.org/10.1007/s10815-020-02037-5
Sakhil HAAl, Rahim AI, Abd Hussein LA. What is the relationship between Sperm DNA integration and Sperm chromatin condensation and their Relation to sperm parameters? Annals of the Romanian Society for Cell Biology. 2021;25(6):17590–5.
https://annalsofrscb.ro/index.php/journal/article/view/9089
Selvam MKP, Agarwal A. A systematic review on sperm DNA fragmentation in male factor infertility: Laboratory assessment. Arab Journal of Urology. 2018;16(1):65–76. https://doi.org/10.1016/j.aju.2017.12.001
Showell MG, Mackenzie‐Proctor R, Brown J, Yazdani A, Stankiewicz MT, Hart RJ. Antioxidants for male subfertility. Cochrane Database of Systematic Reviews [Internet]. 2014 [cited 2023 Jul 20];(12). https://doi.org/10.1002/14651858.CD007411.pub3
Siddhartha N, Reddy NS, Pandurangi M, Muthusamy T, Vembu R, Kasinathan K. The Effect of Sperm DNA Fragmentation Index on the Outcome of Intrauterine Insemination and Intracytoplasmic Sperm Injection. J Hum Reprod Sci. 2019;12(3):189–98. https://doi.org/10.4103%2Fjhrs.JHRS_22_19
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Copyright (c) 2023 Hamdya Aziz, Ula Al-Kawaz, Wasan Adnan Abdulhamed
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